mitochondrial complex iv activity Search Results


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Fig. 5 Measurement of reactive oxygen species (ROS) in myoblasts. (A) Superoxide in <t>mitochondria</t> measured using MitoSox. Left: for reference, wild-type young myoblasts were treated with the increasing concentrations of rotenone (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type). (B) Intracellular ROS including hydrogen peroxide (H2O2) and hydroxyl radical (HO·) measured using CM-H2DCFH fluorescence. Left: for reference, intracellular ROS of wild-type young myoblasts treated with increasing concentrations of BSO (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type).
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complex iv  (Elabscience Biotechnology)
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Fig. 5 Measurement of reactive oxygen species (ROS) in myoblasts. (A) Superoxide in <t>mitochondria</t> measured using MitoSox. Left: for reference, wild-type young myoblasts were treated with the increasing concentrations of rotenone (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type). (B) Intracellular ROS including hydrogen peroxide (H2O2) and hydroxyl radical (HO·) measured using CM-H2DCFH fluorescence. Left: for reference, intracellular ROS of wild-type young myoblasts treated with increasing concentrations of BSO (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type).
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Fig. 5 Measurement of reactive oxygen species (ROS) in myoblasts. (A) Superoxide in <t>mitochondria</t> measured using MitoSox. Left: for reference, wild-type young myoblasts were treated with the increasing concentrations of rotenone (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type). (B) Intracellular ROS including hydrogen peroxide (H2O2) and hydroxyl radical (HO·) measured using CM-H2DCFH fluorescence. Left: for reference, intracellular ROS of wild-type young myoblasts treated with increasing concentrations of BSO (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type).
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mitochondrial complex v activity assay kit  (Novus Biologicals)
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Fig. 5 Measurement of reactive oxygen species (ROS) in myoblasts. (A) Superoxide in <t>mitochondria</t> measured using MitoSox. Left: for reference, wild-type young myoblasts were treated with the increasing concentrations of rotenone (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type). (B) Intracellular ROS including hydrogen peroxide (H2O2) and hydroxyl radical (HO·) measured using CM-H2DCFH fluorescence. Left: for reference, intracellular ROS of wild-type young myoblasts treated with increasing concentrations of BSO (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type).
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Fig. 5 Measurement of reactive oxygen species (ROS) in myoblasts. (A) Superoxide in mitochondria measured using MitoSox. Left: for reference, wild-type young myoblasts were treated with the increasing concentrations of rotenone (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type). (B) Intracellular ROS including hydrogen peroxide (H2O2) and hydroxyl radical (HO·) measured using CM-H2DCFH fluorescence. Left: for reference, intracellular ROS of wild-type young myoblasts treated with increasing concentrations of BSO (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type).

Journal: Aging cell

Article Title: Sod2 overexpression preserves myoblast mitochondrial mass and function, but not muscle mass with aging.

doi: 10.1111/j.1474-9726.2009.00477.x

Figure Lengend Snippet: Fig. 5 Measurement of reactive oxygen species (ROS) in myoblasts. (A) Superoxide in mitochondria measured using MitoSox. Left: for reference, wild-type young myoblasts were treated with the increasing concentrations of rotenone (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type). (B) Intracellular ROS including hydrogen peroxide (H2O2) and hydroxyl radical (HO·) measured using CM-H2DCFH fluorescence. Left: for reference, intracellular ROS of wild-type young myoblasts treated with increasing concentrations of BSO (*P < 0.05 compared to wild-type young; **P < 0.05 compared to aged wild-type).

Article Snippet: The mitochondrial fraction was extracted from myoblasts grown to confluency on collagen-coated 150-mm dishes using a commercial mitochondria extraction kit (Imgenex, San Diego, CA, USA).

Techniques: